goat anti tnfr1  (R&D Systems)

Journal: Cardiovascular Toxicology

Article Title: Simvastatin Significantly Reduced Alcohol-Induced Cardiac Damage in Adolescent Mice

doi: 10.1007/s12012-023-09821-6

Figure Lengend Snippet: Representative photomicrographs of the TNF-α-immunolabelled sections a at a low magnification (Scale bar = 50 µm), b at a high magnification (Scale bar = 50 µm), and c the box plot of the percentage TNF-α distribution across the different experimental groups, for both sexes. Varying degrees of TNF-α expression are noticeable across the experimental groups. In both sexes, TNF-α distribution was significantly higher in the ALC group than in the NT group, thus confirming alcohol toxicity in the cardiomyocytes. Both concentrations of Simvastatin were effective against alcohol-induced myocardial inflammation. A lower Simvastatin concentration was effective in the females but not in the males—an indication of possible sex-specific differences in the effect of Simvastatin against alcohol effects on the cardiomyocytes. No editing was done to the images. NT non-treatment, SIM 5-mg Simvastatin, ALC alcohol, ALC + SIM5 5-mg Simvastatin and alcohol, ALC + SIM15 15-mg Simvastatin and alcohol, NS not significant at P > 0.05

Article Snippet: Serial sections were prepared for haematoxylin and eosin (H&E) staining (for general morphology and morphometry of cardiomyocytes), Masson’s trichrome (MT) staining (for evaluating myocardial fibrosis), or TNF-α immunolabelling (for quantifying the expression of TNF-α; primary antibody—1:250, mouse anti-TNF-α, ab220210, Abcam; secondary antibody—1:1000, goat anti-mouse IgG, BA-9200–1.5, Vector labs).

Techniques: Expressing, Concentration Assay

Journal: Cardiovascular Toxicology

Article Title: Simvastatin Significantly Reduced Alcohol-Induced Cardiac Damage in Adolescent Mice

doi: 10.1007/s12012-023-09821-6

Figure Lengend Snippet: Summary of the morphometries of the cardiomyocyte area and the diameter and the distributions of collagen and TNF-α

Article Snippet: Serial sections were prepared for haematoxylin and eosin (H&E) staining (for general morphology and morphometry of cardiomyocytes), Masson’s trichrome (MT) staining (for evaluating myocardial fibrosis), or TNF-α immunolabelling (for quantifying the expression of TNF-α; primary antibody—1:250, mouse anti-TNF-α, ab220210, Abcam; secondary antibody—1:1000, goat anti-mouse IgG, BA-9200–1.5, Vector labs).

Techniques:

Journal: Microorganisms

Article Title: Adenoviral Vector Codifying for TNF as a Co-Adjuvant Therapy against Multi-Drug-Resistant Tuberculosis

doi: 10.3390/microorganisms11122934

Figure Lengend Snippet: Gene expression of pro-inflammatory cytokines in the different experimental groups. Gene expression quantification by qPCR using the 2^ (−ΔCt) method relative to Rplp0 (housekeeping gene) of ( A ) Ifng , ( B ) Tnf and ( C ) Nos2 at day 14, 28 and 60 of treatment with AdTNF, AdGFP or SS. At day 60 of treatment, a significant increase in gene expression was induced by AdTNF treatment. Asterisks represent statistical significance (**** p < 0.0001, two-way ANOVA).

Article Snippet: Cytokine detection was performed with goat anti-mouse polyclonal antibodies against TNF, IFN-γ and iNOS (Santa Cruz Biotechnology, Santa Cruz, CA, USA), secondary anti-goat antibody-HRP (Goat-on-Rodent HRP-Polymer, Biocare Medical); finally, slides were revealed with diaminobenzidine/H 2 O 2 and contrasted with hematoxylin stain.

Techniques: Gene Expression

Journal: Microorganisms

Article Title: Adenoviral Vector Codifying for TNF as a Co-Adjuvant Therapy against Multi-Drug-Resistant Tuberculosis

doi: 10.3390/microorganisms11122934

Figure Lengend Snippet: Percentage of immunostained cells to pro-inflammatory cytokines and iNOS in pneumonic areas of the different experimental groups. Percentage of immunostained cells to ( A ) IFN-γ, ( B ) TNF and ( C ) iNOS in the pneumonic areas of mice infected with MDR-TB after the indicated days of treatment with one dose of AdTNF, AdGFP or SS administered by intratracheal route. AdTNF induced a significant increment of immunostained cells in all the evaluated time points. Asterisks represent statistical significance (**** p < 0.0001, two-way ANOVA). ( D ) Representative microphags (40×) of immunostaining detection (brown cytoplasm peroxidase staining) of the indicated cytokine and iNOS in pneunmonic areas comparing AdTNF and AdGFP.

Article Snippet: Cytokine detection was performed with goat anti-mouse polyclonal antibodies against TNF, IFN-γ and iNOS (Santa Cruz Biotechnology, Santa Cruz, CA, USA), secondary anti-goat antibody-HRP (Goat-on-Rodent HRP-Polymer, Biocare Medical); finally, slides were revealed with diaminobenzidine/H 2 O 2 and contrasted with hematoxylin stain.

Techniques: Infection, Immunostaining, Staining